LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1859..1955)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1970..1989)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1997..2013
                     /note="M13 Reverse priming site"
     gene            2126..2935
                     /note="Kanamycin resistance gene"
     rep_origin      3056..3729
                     /note="pUC origin"
     vector          join(1867..3732,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1852
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:29648 IMAGE:4562507"
                     /tissue_type="Kidney, renal cell adenocarcinoma"
                     /clone_lib="NIH_MGC_14"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            692..1852
                     /gene="GAL3ST1"
                     /gene_synonym=CST
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:24240"
                     /db_xref="MIM:602300"
     CDS             692..1852
                     /dnas_title="galactose-3-O-sulfotransferase 1"
                     /gene="GAL3ST1"
                     /gene_synonym=CST
                     /codon_start=1
                     /product="galactose-3-O-sulfotransferase 1"
                     /protein_id="AAH19077.1"
                     /db_xref="GI:17512187"
                     /db_xref="GeneID:9514"
                     /db_xref="HGNC:24240"
                     /db_xref="MIM:602300"
                     /translation="MLPPQKKPWESMAKGLVLGALFTSFLLLMYSYAVPPLHAGLAST
                     TPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKH
                     RLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFI
                     TVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLL
                     FFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYF
                     KLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAAL
                     RHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRR
                     MLTPEIQYLMDLGANLWVTKLWKFIRDFLRW"
     misc_feature    692..1852
                     /note="GAL3ST1 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccatgccggc ctggcctcca cgaccccgga
        721 ggccgcagcg tcctgctctc cacctgcact cgagccagag gcagtgatcc gggccaacgg
        781 ctcggcgggg gagtgccagc cgcggcgcaa catcgtgttc ttgaagacgc acaagacggc
        841 cagcagcacc ctgctcaaca tcctgttccg cttcggccag aagcaccggc tcaagttcgc
        901 cttccctaac ggccgcaatg acttcgacta cccgaccttc ttcgcccgca gcctggtgca
        961 ggactatcgg cccggggcct gcttcaacat catctgcaac cacatgcgct tccactacga
       1021 cgaggtgcgc ggcctggtgc cgaccaacgc catcttcatc acggtgctcc gcgaccccgc
       1081 ccgcttgttc gagtcctcct tccactactt cgggccggtg gtgcccctca cgtggaagct
       1141 ctcggccggc gacaagctga ccgagttcct gcaagacccg gatcgctact acgaccccaa
       1201 cggcttcaat gcccactacc tccgaaacct gctcttcttc gacctgggct atgacaacag
       1261 cctggacccc agcagcccgc aggtgcagga gcacatcctg gaggtggagc gtcgcttcca
       1321 cctggtgctc cttcaagagt acttcgacga gtcgctggtg ctgctgaagg acctgctgtg
       1381 ctgggagctg gaggacgtgc tctacttcaa gctcaacgcc cgccgcgact cgcccgtgcc
       1441 gcggctctcg ggggagctgt atgggcgcgc caccgcctgg aacatgctgg actcccacct
       1501 ctaccgccac ttcaacgcca gcttctggcg caaggtggag gccttcgggc gggagcgcat
       1561 ggcccgcgag gtggccgccc tgcgccatgc caacgagcgc atgcggacca tctgcatcga
       1621 cgggggccac gccgtggacg ccgccgccat ccaggacgag gccatgcagc cctggcagcc
       1681 gctgggcacc aagtccatcc tgggctacaa cctcaagaag agcatcgggc agcggcacgc
       1741 gcagctctgc cggcgcatgc tcacgcccga gatccagtac ctgatggacc tcggcgccaa
       1801 cctgtgggtc accaagctct ggaagttcat tcgcgatttc ctgcggtggt gaTAGGACCC
       1861 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA
       1921 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG
       1981 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT
       2041 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA
       2101 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG
       2161 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG
       2221 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT
       2281 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG
       2341 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC
       2401 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC
       2461 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT
       2521 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC
       2581 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC
       2641 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT
       2701 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG
       2761 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA
       2821 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA
       2881 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA
       2941 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG
       3001 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT
       3061 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
       3121 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
       3181 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
       3241 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
       3301 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
       3361 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
       3421 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
       3481 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
       3541 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
       3601 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
       3661 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
       3721 TGCTCACATG TT
//